Here are some studiesabout 2-Methyl-4-Isothiazoline-3-one in different aspects, it may help you make a clear understanding about this chemical.
Irritation (skin)
2-Methyl-4-isothiazolin-3-one(CAS NO:2682-20-4) was applied undiluted by a single application of 0.5 ml to theshaved intact skin of 7 New Zealand White rabbits. Contact time was 3 minutes for 5 animals, 1-hr (and 3 min.) for one animal, 4-hrs for one animal. The application sites were semi-occluded.
After removal of the patch, the animals were observed for 14 days for signs of irritation.No mortality of clinical sign of systemic toxicity were observed during the study.On the 4 and 1 hr sites, skin irritation indicative of corrosivity was observed on day 14 and 7,respectively.
Human study
The cumulative irritation potential of 2-Methyl-4-isothiazolin-3-one was -investigated in 21 day test with human volunteers. Aqueous dilutions of MI wereapplied to the back under occlusive patches, for a contact period of 23 hours, on 21 consecutivedays. On completion of the dosing phase, the subjects were rested without further dosing for 10 -14 days.
Following the rest period, 24 hour patch(es) of the appropriate test material(s) were applied to a naïve site. Subject induced with 50, 100 and 250 ppm MI were challenged with the same respective concentrations of test material as well as distilled water and sodium lauryl sulphate. Subjects induced with 500 ppm MI were challenged with 100, 250 and 500 ppm MI as well as with distilled water and SLS. Four of the subjects induced with 1000 ppm were not only challenged with 1000 ppm but also with 250 and 500 ppm.
Irritation (mucous membranes)
2-Methyl-4-Isothiazoline-3-one was applied to the conjunctival sac of a group of six New Zealand White rabbits, as a 100 ppm aqueous solution. Eye irritation was evaluated according to Draize criteria at approximately 1, 24, 48, and 72 hours. After the 24 hour observation, each eye (treated and control) was irrigated with 0.9% saline for approximately 60 seconds. No mortality or clinical signs of systemic toxicity were observed. No corneal, conjunctival or iridial effects were observed during the study
Sensitisation
The skin sensitisation potential of 2-Methyl-4-isothiazolin-3-one (CAS NO:2682-20-4) was determined using the closed patch method of Buehler. Four groups of outbred Hartley guinea pigs received ten 6-hr induction doses of 0.4 ml at concentrations of 1000, 5000, 15,000 and 30,000 ppm a.i. in distilled water.
Two weeks after the last induction dose these animals, together with a group of uninduced control
animals, were challenged with 1000, 5000, or 15,000 ppm MI in distilled water. No erythema reactions were observed in the non-induced control animals at any challenge concentration of 2-Methyl-4-isothiazolin-3-one.
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